With each eye movement, stationary objects in the world change position on the retina yet we perceive the world as stable. Spatial updating is one neural mechanism by which the brain compensates for shifts in the retinal image. The brain circuits that produce updated spatial representations remain unknown. We tested the hypothesis that the corpus callosum is required for updating visual information from one hemisphere to the other in humans. We used fMRI to measure activity related to spatial updating in four neurologically intact individuals and one split-brain subject (ML) who had undergone a complete callosotomy fifteen years prior to the study. Subjects performed a single-step saccade task that we have used previously to demonstrate spatial updating in human parietal cortex. In this task, the subject fixates a cross while a salient visual stimulus flickers in the periphery. The stimulus is then extinguished and a tone cues the subject to make a horizontal saccade to a second cross. The size and direction of the saccade are selected so as to bring the location of the preceding stimulus into the opposite hemifield. Spatial updating is observed as the shift in activity from one hemisphere to the other in conjunction with the saccade. We observed robust spatial updating in parietal cortex in ML and in the neurologically intact individuals. This result demonstrates that spatial representations can be updated across visual hemifields in the absence of the corpus callosum in humans.